Abstract： Objective:To review the HIV confirmatory test results for screening reactive samples in Tongzhou district of Beijing from 2017 to 2021 and understand the situation of HIV/AIDS epidemics in the district, so as to provide data support for HIV/AIDS prevention and control.Methods:Western blot was used as the confirmatory test for HIV screening reactive samples in Tongzhou district from 2017 to 2021. The test results were analyzed according to gender, age, submitting institution, detection route and other categories.Results:During 2017-2021, 2 480 samples were received in the confirmatory laboratory of Tongzhou district, including 994 (40.1%) anti-HIV-1 antibody positive samples, 774 (31.2%) HIV indeterminate samples and 712 (28.7%) negative samples. The confirmatory positive rate was higher in male (51.6%) than that in female (8.6%) and the rate in 20-39 years old group (44.4%) was higher than other age groups. The main route of infection among positive cases was sexual transmission (93.7%). The confirmatory positive rate of samples from blood center was the lowest (10.5%) while the samples from CDC had the highest rate (91.8%). The differences of confirmatory positive rates in residence registration, complication with syphilis and sample testing routes were statistically significant ( P<0.05). The differences of confirmatory positive rates in screening laboratories (46.8%) and rapid detection points (47.0%) were not statistically significant. The most common WB band pattern in anti-HIV-1 antibody positive samples was presence of all bands (89.5%). The HIV indeterminate samples presented mainly single band (70.8%). Conclusions:Strengthening further the propagation of HIV/AIDS prevention and control, expanding HIV antibody testing and increasing follow-up of HIV indeterminate persons would be necessary to promote the early detection and treatment of HIV infection.