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目的 研究转录相关锌带蛋白基因(ZNRD1)在肾癌及肾脏正常组织巾的表达.方法 应用免疫组化法,检测71例肾癌组织和24例癌旁正常肾脏组织中ZNRD1蛋白的表达,并分析ZNRD1蛋白表达与肾癌Gleason分级和临床分期的关系.应用逆转录聚合酶链反应(RT-PCR)和Western blot法,分别检测20例肾癌及痛旁正常肾脏组织中ZNRD1 mRNA和蛋白的表达.结果 免疫组化染色结果 显示,ZNRD1蛋白主要定位于细胞核.ZNRD1蛋白在24例肾癌及其相应的癌旁正常肾脏组织中的阳性表达率分别为91.7%(22/24)和20.8%(5/24),差异具有明显的统计学意义(P＜0.01).ZNRD1蛋白的表达与肾癌Gleason分级和临床分期均无显著的相关性(均P＞0.05).RT-PCR检测结果 显示,ZNRD1 mRNA在肾癌组织中的相对表达量为0.6186,明显高于癌旁正常肾脏组织(0.4273,P＜0.01).Western blot检测结果 湿示,ZRND1蛋白在肾癌组织中的相对表达量为0.5623,亦明显高于癌旁正常肾脏组织(0.3885,P＜0.01).结论 ZNRD1可能在肾癌的发生发展中发挥着重要作用.

Objective To investigate the expression of zinc ribbon domain-containing1(ZNRD1)in human renal cell carcinonla and normal kidney tissues.Methods ThC expression of ZNRD1 protein was examined by immunohistochemical staining in 71 renal cell carcinomas and 24 samples of normal kidney tissue.The correlation between the expressions of ZNRD1 protein and clinicopathologic features was analyzed.The expression of ZNRD1 mRNA and ZNRD1 protein Was detected by quantitative reverse transeriptase-polymerase chain reaction(PT-PCR)and Western blot in 20 renal cell carcinomas and corresponding adjacent non-cancerous tissues.Results ZNRD1 protein Was detected mostly in the cell nuclei by immunohistochemistry.The positive expression rate of ZNRD1 protein was 91.7%(22/24)in renal cell carcinomas and 20.8%(5/24)in the normal kidney tissues,with a statistically significant difference between cancer and normal kidney tissue(P＜0.01).However,no significant correlation was observed between ZNRD1 protein expression level and clinicopathologic features(P＞0.05).ZNRD1 mRNA expression level was significantly higher in renal cell carcinomas(0.6186)than that in the normal kidney tissues(0.4273)assessed by RT-PCR(P＜0.01).The expression level of ZNRDl protein by Western blot was 0.5623 in renal cell carcinomas.significantly higher than that in normal kidney tissues (0.3885,P＜0.01).Conclusion ZNRD1 gene and ZNRD1 protein may play an important role in the carcinogenesis of renal cell carcinoma.Further investigation is still needed.