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目的 研究NQO1基因多态性和环境因素的交互作用与肝细胞癌易感性的关系. 方法 采用以manbet官网登录 为基础的病例对照研究方法,运用TaqMan MGB荧光定量实时PCR分析方法对病例组400例肝细胞癌患者和对照组400例非肿瘤患者进行NQO1基因C609T位点的基因型分析.以非条件logistic回归模型分析比较各基因型在两组中分布频率的差异,以及基因多态性和环境因素的交互作用.结果 NQO1基因C609T位点CC、CT和TT各基因型频率分别为23.75％、50.25％和28.00％,对照组中3种基因型频率分别为37.55％、43.75％和18.25％,差异有统计学意义(P＜0.05).与CC基因型相比,CT或者TT基因型的个体罹患HCC的风险OR分别为2.106(95％CI:1.137～3.110)和2.564(95％CI:1.357～4.744).T等位基因携带者患肝细胞癌的危险性是C等位基因携带者的1.86倍(OR=1.86,95％ CI:1.235 ～ 2.980).交互作用分析结果表明NQO1基因多态性与肿瘤家族史、HBsAg阳性之间在肝细胞癌发生中存在交互作用,交互作用的OR值分别为2.431、8.359. 结论 NQO1C609T基因型可能是广西南部地区人群患肝细胞癌的危险因素之一,NQO1基因多态性与肿瘤家族史、HBsAg阳性之间在肝细胞癌发生中存在交互作用,能增加罹患肝细胞癌的风险.

Objective To study the relationship between hepatocellular carcinoma (HCC) and the interaction of polymorphisms in the NAD(P)H:quinone oxidoreductase (NQO1) gene with environmental factors using a hospital-based case-control study.Methods Four-hundred newly diagnosed HCC cases and 400 healthy individuals (non-tumor controls) were enrolled in the study.Demographic information and medical history was obtained by questionnaire.TaqMan minor groove binder real-time PCR was carried out to detect the NQO1 C609T genotype using blood-derived DNA from all study participants.Unconditional logistic regression analysis was carried out to estimate the odds ratios (ORs) and 95％ confidence intervals (CIs).Results The frequencies of NQO1 609 CC,CT and TT genotypes were 23.75％,50.25％ and 28.00％ in the HCC group,and 37.55％,43.75％ and 18.25％ in the control group.The differences between the HCC and control group reached statistical significance (all P ＜ 0.05).The ORs of NQO1 609 CT and TT genotypes were significantly higher compared to the CC genotype; the adjusted OR(95％ CI) of CT was 2.106(1.137-3.110) and of TT was 2.564(1.357-4.744).Individuals carrying the NQO1 609 T allelic gene had a significantly higher risk of HCC than those carrying the C allelic gene; the adjusted OR(95％ CI) was 1.86(1.235-2.980).Interactions were found between hepatitis B virus infection with hepatitis B surface antigen (HBsAg)-positivity and NQO 1 gene polymorphisms (adjusted OR:2.431) and history of cancer (adjusted OR:8.3592).Conclusion The NQO1 C609T genotype is associated with increased risk of HCC.Interactions between HBsAg-positive infection,history of cancer,and NQO1 gene polymorphisms may contribute to HCC.