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目的：探讨磷脂酶Cε1（PLCE1）在胃癌组织中的表达及其临床意义。方法收集浙江省人民manbet官网登录 2005年1月至2007年12月间125例胃癌手术标本及其癌旁胃黏膜组织，应用免疫组织化学染色方法检测PLCE1在胃癌及癌旁组织中的表达，并通过实时荧光定量PCR检测其中41例胃癌及其癌旁组织中PLCE1 mRNA表达水平。分析PLCE1蛋白表达与胃癌临床病理特征及预后的关系。结果 PLCE1蛋白在胃癌和癌旁组织中的阳性表达率分别为53．6％（67/125）和24．0％（30/125），差异有统计学意义（P＜0．01）；在65．9％（27/41）的胃癌组织中PLCE1 mRNA表达水平较癌旁胃黏膜上调2倍以上。 PLCE1蛋白表达与胃癌分化程度、浸润深度、淋巴结转移、远处转移及TNM分期有关（均P＜0．01）。 PLCE1阴性与阳性表达者5年总体生存率分别为54．0％和31．2％，差异有统计学意义（P＜0．01）；但多因素分析显示，PLCE1表达并不是影响胃癌患者预后的独立因素（P＞0．05）。结论 PLCE1在胃癌组织中高表达，且与胃癌的恶性生物学行为相关。

Objective To study the expression of phospholipase C epsilon-1 (PLCE1) and its clinical significance in gastric cancer. Methods Surgical specimens were collected from 125 patients who underwent radical gastrectomy between 2005 and 2007 in the Zhejiang Provincial Peoples′Hospital. Expression level of PLCE1 protein was measured by immunohistochemistry in these 125 surgical specimens, which included primary gastric cancer and matched adjacent normal gastric mucosa tissues, and then 41 pairs of above specimens were selected randomly to examine the expression level of PLCE1 mRNA by quantitative reverse transcription PCR (qRT-PCR). Results Immunohistochemistry and qRT-PCR showed that both protein and mRNA level of PLCE1 were up-regulated in gastric cancer compared with paired normal gastric mucosa. Univariate analysis demonstrated that the expression of PLCE1 was significantly associated with differentiation degree, invasion depth, lymph node metastasis, distant metastasis, and TNM stage (all P<0.01). The 5-year survival rate of positive PLCE1 group was significantly lower as compared to negative group (31.2%vs. 54.0%, P<0.01). However, the expression of PLCE1 was not an independent prognostic factor for gastric cancer (P>0.05). Conclusions PLCE1 is up-regulated in gastric cancer,which is associated with the malignant biological behaviors of gastric cancer. High expression of PLCE1 suggests poor prognosis.